Tet-on, or Tet-off, that is the question: Advanced conditional gene expression in Aspergillus
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- 作者：Franziska Wankaa ; franziska.wanka@tu-berlin.de" class="auth_mail" title="E-mail the corresponding author ; Timothy Cairnsa ; t.cairns@campus.tu-berlin.de" class="auth_mail" title="E-mail the corresponding author ; Simon Boeckera ; simon.boecker@chem.tu-berlin.de" class="auth_mail" title="E-mail the corresponding author ; Christian Berensb ; christian.berens@fli.bund.de" class="auth_mail" title="E-mail the corresponding author ; Anna Happelc ; anna.happel@med.stud.uni-erlangen.de" class="auth_mail" title="E-mail the corresponding author ; Xiaomei Zhengd ; zheng_xm@tib.cas.cn" class="auth_mail" title="E-mail the corresponding author ; Jibin Sund ; sun_jb@tib.cas.cn" class="auth_mail" title="E-mail the corresponding author ; Sven Krappmannc ; sven.krappmann@uk-erlangen.de" class="auth_mail" title="E-mail the corresponding author ; Vera Meyera ; vera.meyer@tu-berlin.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Aspergillus ; Tet-on ; Tet-off ; Conditional expression ; Heterologous gene expression ; Genetic engineering
- 刊名：Fungal Genetics and Biology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：89
- 期：Complete
- 页码：72-83
- 全文大小：2392 K
文摘
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A titratable Tet-off system has been developed for Aspergillus.
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Tet-off enables both gene silencing and over-expression in a single strain.
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Genetic stability of the Tet-on system has been improved for A. niger.
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Modified luciferase reporters enable the study of gene oscillations.
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The approaches described can broadly be applied to other filamentous fungi.