An electrochemical biosensor for double-stranded Wnt7B gene detection based on enzymatic isothermal amplification
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- 作者：Junlong Lia ; Zhongping Chenb ; Yu Xiangc ; Lili Zhoua ; Ting Wanga ; Zhang Zhanga ; Kexin Suna ; Dan Yina ; Yi Lia ; Guoming Xiea ; guomingxie@cqmu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; xiegm059@foxmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Electrochemical biosensor ; Double-stranded DNA detection ; Isothermal amplification ; Wnt7B gene
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 December 2016
- 年：2016
- 卷：86
- 期：Complete
- 页码：75-82
- 全文大小：2330 K
文摘
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ssDNA produced from dsDNA target using nicking enzyme exploited and polymerase extension.
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A low DNA content could be detect due to the isothermal amplification process.
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Both the specific recognition of nicking enzyme and hairpin self-assembly triggered recycling ensured the high selectivity of this method.
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The proposed strategy could be extended to the detection of other DNA by simply exchanging the corresponding DNA sequence.