Distinct metabolic effects of resveratrol on lipogenesis markers in mice adipose tissue treated with high-polyunsaturated fat and high-protein diets

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• 作者：Keila Lopes Mendes^a ; ^b ; Luciné ; ia de Pinho^a ; ^c ; Joã ; o Marcus Oliveira Andrade^a ; Alanna Fernandes Paraí ; so^a ; Jamille Fernandes Lula^a ; Simone Moreira Macedo^a ; John David Feltenberger^d ; André ; Luiz Sena Guimarã ; es^a ; Alfredo Maurí ; cio Batista de Paula^a ; Sé ; rgio Henrique Sousa Santos^a ; ^e ; ^{sergiosousas@hotmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Resveratrol (PubChem CID ; 445154)
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：15 May 2016
• 年：2016
• 卷：153
• 期：Complete
• 页码：66-73
• 全文大小：980 K

文摘

A healthy diet is essential for the prevention of metabolic syndrome. The present study evaluated the effect of resveratrol associated with high-polyunsaturated fat and high-protein diets on expression of adipogenic and lipogenic genes.

Research methods & procedures

FVB/N mice were divided into 6 groups (n = 7 each) and fed with experimental diets for 60 days: standard (ST), high-fat diet (HFD), and high-protein diet (HPD), with and without resveratrol (RSV) (4 g/kg diet). The body weight, food intake, energy intake (kcal), and blood parameters (HDL-C, total cholesterol, glucose, and triglyceride levels) were assessed. Real-time PCR was performed to analyze the expression of adipogenesis and lipogenesis markers: PPARγ, SREBP-1c, ACC and FAS in samples from perigonadal adipose tissue.

Results

In the HPD + RSV group, resveratrol decreased body weight, body adiposity, adipose tissue weight, adipocyte area, total cholesterol, ACC and FAS expression, and increased HDL-cholesterol in comparison to HPD. In the HPD group there was a decrease in adipocyte area, as well as PPARγ, SREBP-1c and ACC expression in comparison to ST. While in HFD + RSV, resveratrol decreased levels of total cholesterol in comparison to HFD. In the HFD group there was decrease in body weight, and PPARγ, SREBP-1c and ACC expression in comparison to ST.

Conclusions

The obtained results show that resveratrol decreases lipogenesis markers and metabolic parameters in the setting of a high-protein diet. Moreover, resveratrol decreased total cholesterol in both diets. These results point to the increased potential of resveratrol use in prevention of metabolic syndrome, acting on different dietary compositions.