Detection of treatment-resistant infectious HIV after genome-directed antiviral endonuclease therapy
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- 作者：Harshana S. De Silva Feelixgea ; 1 ; hdesilva@fredhutch.org" class="auth_mail" title="E-mail the corresponding author ; Daniel Stonea ; 1 ; dstone2@fredhutch.org" class="auth_mail" title="E-mail the corresponding author ; Harlan L. Pietza ; c ; d ; 2 ; harlan.pietz@nih.gov" class="auth_mail" title="E-mail the corresponding author ; Pavitra Roychoudhurya ; proychou@fredhutch.org" class="auth_mail" title="E-mail the corresponding author ; Alex L. Greningerb ; agrening@uw.edu" class="auth_mail" title="E-mail the corresponding author ; Joshua T. Schiffera ; e ; jschiffe@fredhutch.org" class="auth_mail" title="E-mail the corresponding author ; Martine Auberta ; maubert@fredhutch.org" class="auth_mail" title="E-mail the corresponding author ; Keith R. Jeromea ; b ; c ; kjerome@fredhutch.org" class="auth_mail" title="E-mail the corresponding author ; kjerome@fhcrc.org" class="auth_mail" title="E-mail the corresponding author
- 关键词：Zinc finger nuclease ; Endonuclease ; Resistance ; Reverse transcriptase
- 刊名：Antiviral Research
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：126
- 期：Complete
- 页码：90-98
- 全文大小：2011 K
文摘
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Zinc finger nucleases (ZFNs) that cleave HIV pol were developed as antiviral agents.
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ZFNs could inhibit production of HIV virions and infectious HIV in vitro.
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An infectious and ZFN-resistant HIV provirus was identified in treated cells.
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ZFN-resistance HIV remained susceptible to NRTi and NNRTi drugs.
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Secondary mutations rendered the ZFN-resistance virus replication incompetent.