Development of a new HPLC method using fluorescence detection without derivatization for determining purine nucleoside phosphorylase activity in human plasma
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- 作者：Patricia Giuliani ; p.giuliani@unich.it" class="auth_mail" title="E-mail the corresponding author ; Mariachiara Zuccarini mariachiarazuccarini@libero.it" class="auth_mail" title="E-mail the corresponding author ; Silvana Buccella sbuccella@unich.it" class="auth_mail" title="E-mail the corresponding author ; Margherita Rossini margherita.rossini@unich.it" class="auth_mail" title="E-mail the corresponding author ; Iolanda D&rsquo ; Alimonte i.dalimonte@unich.it" class="auth_mail" title="E-mail the corresponding author ; Renata Ciccarelli r.ciccarelli@unich.it" class="auth_mail" title="E-mail the corresponding author ; Matteo Marzo matteo.marzo@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Antonio Marzo antoniop.marzo@libero.it" class="auth_mail" title="E-mail the corresponding author ; Patrizia Di Iorio pat.diiorio@unich.it" class="auth_mail" title="E-mail the corresponding author ; Francesco Caciagli f.caciagli@unich.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：PNP ; purine nucleoside phosphorylase ; Pi ; inorganic phosphate ; INO ; inosine ; GUO ; guanosine ; UA ; uric acid ; HYPO ; hypoxanthine ; GUA ; guanine ; XAN ; xanthine ; NaOH ; sodium hydroxide ; K2HPO4 ; dipotassium phosphate ; KH2PO4 ; monopotassium phosphate ; HEPES ; 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid ; DAD ; diode array detector ; PFP ; pentafluorophenyl ; QC ; quality control ; LOD ; limit of detection ; LOQ ; limit of quantification ; r ; correlation coefficient
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：1009-1010
- 期：Complete
- 页码：114-121
- 全文大小：1298 K
文摘
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HPLC method combining fluorescence and UV detection for measuring purine metabolites.
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The method was validated and proved to be fast, sensitive, specific.
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The Fluorescence detection improves the limit of detection of guanosine and guanine.
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Kinetic parameters of human plasma PNP were determined.
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This method is suitable for routine evaluation of PNP activity as possible biomarker.