Poly-l-lysine/heparin multilayer coatings prevent blood protein adsorption

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• 作者：Alejandro Barrantes^a ; ^b ; ^{a.b.bautista@odont.uio.no" class="auth_mail" title="E-mail the corresponding author} ; ^{barrantes_ale@yahoo.es" class="auth_mail" title="E-mail the corresponding author} ; Jonas Wengenroth^a ; ^{jonas.wengenroth@odont.uio.no" class="auth_mail" title="E-mail the corresponding author} ; Thomas Arnebrant^b ; ^{thomas.arnebrant@mah.se" class="auth_mail" title="E-mail the corresponding author} ; Hå ; vard J. Haugen^a ; ^{h.j.haugen@odont.uio.no" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Layer by layer ; Protein adsorption ; Ellipsometry ; QCM-D ; Heparin coating
• 刊名：Journal of Colloid and Interface Science
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：485
• 期：Complete
• 页码：288-295
• 全文大小：1388 K

文摘

The adsorption of blood proteins, serum albumin (BSA), immunoglobulin G (IgG) and fibrinogen (FGN), onto model SiO₂ planar surfaces coated with poly-class="smallcaps">l-lysine/heparin multilayers (PLL/HEP) has been investigated by means of ellipsometry and quartz crystal microbalance with dissipation. Aiming at the development of low fouling coatings, this study has been focused on the effects that the number of layers and the type of polyelectrolyte present on the topmost layer have on the adsorption of these proteins. The three proteins interact with PLL-ended coatings whereas HEP-ended coatings prevent the adsorption of both BSA and IgG and induce a decrease in the adsorbed amount of FGN, down to 0.4 mg/m² for three bilayers, as the number of PLL/HEP bilayers increases. These results suggest that heparin-ended multilayers prevent protein adsorption, which is an indicative of good blood compatibility. As a consequence we propose that PLL/HEP coatings could be used for the development of vascular medical devices.