PICKLE is a CHD subfamily II ATP-dependent chromatin remodeling factor

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• 作者：Kwok Ki Ho ; Heng Zhang ; Barbara L. Golden ; Joe Ogas ; ^{ogas@purdue.edu}
• 关键词：PHD ; plant homeodomain ; PKL ; PICKLE ; HDAC ; histone deacetylase ; MBD2 ; methyl-CpG-binding domain protein 2 ; H3K27me3 ; trimethylation of lysine 27 histone H3 ; LEC ; Leafy Cotyledon ; ACT7 ; Actin7 ; UBQ10 ; Ubiquitin10 ; PKR ; Pickle-related ; DUF ; domain of unknow
• 刊名：Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：1829
• 期：2
• 页码：199-210
• 全文大小：1365 K

文摘

PICKLE plays a critical role in repression of genes that regulate development identity in Arabidopsis thaliana. PICKLE codes for a putative ATP-dependent chromatin remodeler that exhibits sequence similarity to members of subfamily II of animal CHD remodelers, which includes remodelers such as CHD3/Mi-2 that also restrict expression of developmental regulators. Whereas animal CHD3 remodelers are a component of the Mi-2/NuRD complex that promotes histone deacetylation, PICKLE promotes trimethylation of histone H3 lysine 27 suggesting that it acts via a distinct epigenetic pathway. Here, we examine whether PICKLE is also a member of a multisubunit complex and characterize the biochemical properties of recombinant PICKLE protein. Phylogenetic analysis indicates that PICKLE-related proteins in plants share a common ancestor with members of subfamily II of animal CHD remodelers. Biochemical characterization of PICKLE in planta, however, reveals that PICKLE primarily exists as a monomer. Recombinant PICKLE protein is an ATPase that is stimulated by ssDNA and mononucleosomes and binds to both naked DNA and mononucleosomes. Furthermore, recombinant PICKLE exhibits ATP-dependent chromatin remodeling activity. These studies demonstrate that subfamily II CHD proteins in plants, such as PICKLE, retain ATP-dependent chromatin remodeling activity but act through a mechanism that does not involve the ubiquitous Mi-2/NuRD complex.