The Arabidopsis Protein CGLD11 Is Required for Chloroplast ATP Synthase Accumulation

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• 作者：Sabine Grahl¹ ; ⁴ ; ⁵ ; Bennet Reiter² ; ⁵ ; Irene Luise Gü ; gel¹ ; ⁴ ; Evgenia Vamvaka² ; Chiara Gandini² ; Peter Jahns³ ; Jü ; rgen Soll¹ ; ⁴ ; Dario Leister² ; ^{leister@lrz.uni-muenchen.de" class="auth_mail" title="E-mail the corresponding author} ; Thilo Rü ; hle²
• 关键词：chloroplast ; mitochondria ; photosynthesis ; dual targeting ; ATP synthase ; assembly
• 刊名：Molecular Plant
• 出版年：2016
• 出版时间：6 June 2016
• 年：2016
• 卷：9
• 期：6
• 页码：885-899
• 全文大小：2609 K

文摘

ATP synthases in chloroplasts (cpATPase) and mitochondria (mtATPase) are responsible for ATP production during photosynthesis and oxidative phosphorylation, respectively. Both enzymes consist of two multisubunit complexes, the membrane-bound coupling factor O and the soluble coupling factor 1. During cpATPase biosynthesis, several accessory factors facilitate subunit production and orchestrate complex assembly. Here, we describe a new auxiliary protein in Arabidopsis thaliana, which is required for cpATPase accumulation. AtCGLD11 (CONSERVED IN THE GREEN LINEAGE AND DIATOMS 11) is a protein without any known functional domain and shows dual localization to chloroplasts and mitochondria. Loss of AtCGLD11 function results in reduced levels of cpATPase and impaired photosynthetic performance with lower rates of ATP synthesis. In yeast two-hybrid experiments, AtCGLD11 interacts with the β subunits of the cpATPase and mtATPase. Our results suggest that AtCGLD11 functions in F₁ assembly during cpATPase biogenesis, while its role in mtATPase biosynthesis may not, or not yet, be essential.