The plasma membrane protein Rch1 is a negative regulator of cytosolic calcium homeostasis and positively regulated by the calcium/calcineurin signaling pathway in budding yeast

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• 作者：Yunying Zhao^a ; ^b ; Hongbo Yan^a ; Ricardo Happeck^c ; Tina Peiter-Volk^c ; Huihui Xu^a ; Yan Zhang^a ; Edgar Peiter^c ; Chloë ; van Oostende Triplet^d ; Malcolm Whiteway^e ; Linghuo Jiang^a ; ^b ; ^{linghuojiang@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：SLC ; solute carrier family ; CDRE ; calcineurin-dependent response element
• 刊名：European Journal of Cell Biology
• 出版年：2016
• 出版时间：March-May 2016
• 年：2016
• 卷：95
• 期：3-5
• 页码：164-174
• 全文大小：2353 K

文摘

Saccharomyces cerevisiae Rch1 is structurally similar to both the vertebrate solute carrier SLC10A7 and Candida albicans Rch1. We show here that ScRCH1 is a functional homolog of CaRCH1. In S. cerevisiae, overexpression of ScRCH1 suppresses, but deletion of ScRCH1 does not affect, the lithium and rapamycin tolerance of pmr1 cells. Overexpression of ScRCH1 reduces expression of ENA1, prevents sustained accumulation of cytosolic calcium and reduces the activation level of calcium/calcineurin signaling in pmr1 cells. Therefore, similar to the situation in the pathogen C. albicans, ScRch1 negatively regulates the cytosolic homeostasis in response to high levels of extracellular calcium. ScRch1 proteins distribute as multiple foci in the plasma membrane prior to cell division, move toward and concentrate at the bud neck as the bud grows in size, and disperse again along the plasma membrane immediately prior to cytokinesis. Furthermore, our genetic and biochemical data also demonstrate that transcriptional expression of RCH1 is positively regulated by calcium/calcineurin signaling through the sole CDRE element in its promoter.