We report the synthesis and characterization of
multifunctional peptides co
mprised of a hydrogel for
ming β-sheet peptide seg
ment and a
matrix
metalloproteinase 2 substrate containing a propargylglycinyl linker that is further derivatized with an RGD peptide sequence
m>viam> “click” che
mistry. In contrast to currently known syste
ms, these
multifunctional peptides for
med gels that are stiffer than those for
med by their respective precursors. All the peptides showed reversible ther
moresponsive properties, which render the
m as suitable lead syste
ms for a variety of possible bio
medical applications.
Statement of Significance
In general, it has been frequently observed that chemical biofunctionalization of peptide hydrogels adversely affects peptide assembly, hydrogel formation or mechanical properties, which severely compromises their application. A functionalization protocol that allows to generate peptide hydrogels that display significantly improved mechanical properties over their unfunctionalized counterparts is reported in this work. These peptides also showed thermoresponsive viscoelastic characteristics, including an example of a peptide hydrogel that displays lower critical solution temperature behaviour.