Crystal Structure and Role of Glycans and Dimerization in Folding of Neuronal Leucine-Rich Repeat Protein AMIGO-1

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• 作者：Tommi Kajander¹ ;   ; ^&dagger ; ;   ; ^{tommi.kajander@helsinki.fi} ; Juha Kuja-Panula² ;   ; ^&dagger ; ; Heikki Rauvala² ; Adrian Goldman¹
• 关键词：protein structure ; LRR ; protein folding ; adhesion ; glycosylation
• 刊名：Journal of Molecular Biology
• 出版年：2011
• 出版时间：11 November 2011
• 年：2011
• 卷：413
• 期：5
• 页码：1001-1015
• 全文大小：1924 K

文摘

AMIGO-1 is the parent member of a novel family of three cell surface leucine-rich repeat (LRR) proteins. Its expression is induced by the binding of HMGB1 (high-mobility group box 1 protein) to RAGE (receptor for advanced glycation end products) on neurons. Binding of HMGB1 to RAGE is known to have a direct effect on cellular growth regulation and mobility, and AMIGO-1 directly supports growth of neuronal processes and fasciculation of neurites. In addition, the second member of the AMIGO-family, AMIGO-2, has been implicated in adhesion of tumor cells in adenocarcinoma and survival of neurons.

We have determined the crystal structure of AMIGO-1 at 2.0?? resolution, which reveals a typical cell surface LRR domain arrangement with N- and C-terminal capping domains with disulfide bridges, followed by a C2-type Ig domain. AMIGO-1 is a dimer, with the LRR regions forming the dimer interface, and sequence conservation analysis and static light-scattering measurements suggest that all three AMIGO family proteins form similar dimers. Based on the AMIGO-1 structure, we have also modeled AMIGO-2 and present small-angle X-ray scattering data on AMIGO-2 and AMIGO-3. Our mutagenesis studies show that AMIGO-1 dimerization is necessary for proper cell surface expression and thus probably for proper or stable folding in the endoplastic reticulum and for the function of the protein. Based on the data presented earlier, we also suggest that dimerization through the LRR¨CLRR interface is likely to be involved in cell¨Ccell adhesion by AMIGO-1, while extensive glycosylation may have a role.