Hydrogen peroxide derived from marine peroxy sesquiterpenoids induces apoptosis in HCT116 human colon cancer cells

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• 作者：Haruna Miyazato^a ; Junsei Taira^a ; ^{taira@okinawa-ct.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Katsuhiro Ueda^b
• 关键词：Peroxy sesquiterpenoid ; Apoptosis ; Reactive oxygen species ; Hydrogen peroxide ; HCT colon cancer cells
• 刊名：Bioorganic & Medicinal Chemistry Letters
• 出版年：2016
• 出版时间：1 October 2016
• 年：2016
• 卷：26
• 期：19
• 页码：4641-4644
• 全文大小：945 K

文摘

In this study, the isolates of the peroxy sesquiterpenoids (1–3) from the Okinawan soft coral, Sinularia sp., indicated cytotoxicity in HCT116 colon cancer cells. The apoptotic cells with a nuclear condensation were detected in the presence of these compounds, then the caspase 3/7 activity was induced, indicating that the compounds have a potential antitumor activity by apoptosis-induction. The cells treated with these compounds were generated reactive oxygen species (ROS), indicating that the ROS is related to the induction of apoptosis. The ROS production reduced in the presence of catalase or trolox, indicating that hydrogen peroxide (H₂O₂) is generated through a certain free radical reaction derived from the compound. In fact, the accumulation of intracellular H₂O₂ was also confirmed in the presence of these compounds. Based on all the results, this study proposed the apoptosis-inducing mechanism due to the compounds that the H₂O₂ produced involving free radical reactions derived from cleavage of the end or hydro-peroxide in the molecule induced cell death.