Potentiometric sensors doped with biomolecules as a new approach to small molecule/biomolecule binding kinetics analysis

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• 作者：D. Daems ; K. De Wael ; K. Vissenberg ; G. Van Camp ; L. Nagels
• 关键词：Binding kinetics ; Potentiometry ; Biosensor ; Dopamine ; Aptamer
• 刊名：Biosensors and Bioelectronics
• 出版年：15 April, 2014
• 年：2014
• 卷：54
• 期：Complete
• 页码：515-520
• 全文大小：583 K

文摘

The most successful binding kinetics analysis systems at this moment include surface plasmon resonance (SPR), quartz microcrystal balance (QMB) and surface acoustic wave (SAW). Although these are powerful methods, they generally are complex, expensive and require the use of monolayers. Here, we report on potentiometric sensors as an inexpensive and simple alternative to do binding kinetics analysis between small molecules in solution and biomolecules (covalently) attached in a biopolymer sensor coating layer. As an example, dopamine and an anti-dopamine aptamer were used as the small molecule and the biomolecule respectively. Binding between both follows a Langmuir adsorption type model and creates a surface potential. The system operates in Flow Injection Analysis mode (FIA). Besides being an interesting new binding kinetics tool, the approach allows systematic design of potentiometric biosensors (in the present study a dopamine sensor), and gives new insights into the functioning of ion-selective electrodes (ISE's).