HPAEC-PAD quantification of Haemophilus influenzae type b polysaccharide in upstream and downstream samples
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- 作者：Robert M.F. van der Puta ; robert.van.der.put@intravacc.nl" class="auth_mail" title="E-mail the corresponding author ; Alex de Haana ; Jan G.M. van den IJssela ; Ahd Hamidia ; Michel Beurretb ; 2
- 关键词：CPS ; capsular polysaccharide ; DSP ; downstream processing ; ESTD ; external standard ; Glc ; glucose ; Glc-6-P ; d-glucose-6-phosphate ; Hib ; Haemophilus influenzae type b ; HPAEC-PAD ; high performance anion exchange chromatography with pulsed amperometric detection ; HPSEC ; high performance size exclusion chromatography ; IPC ; in process control ; kDa ; kilo Dalton ; μRIU ; micro-refractive index unit ; Mw ; absolute weight-average molecular mass ; MWCO ; molecular weight cut-off ; NA ; nucleic acids ; NaAc ; sodium
- 刊名：Vaccine
- 出版年：2015
- 出版时间：27 November 2015
- 年：2015
- 卷：33
- 期：48
- 页码：6908-6913
- 全文大小：1110 K
文摘
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Hib fermentation (USP) and purification (DSP) products were analyzed by HPAEC-PAD.
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Colorimetric methods are not sufficiently accurate.
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Correlation between HPAEC-PAD and orcinol data was high for DSP samples.
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The HPAEC-PAD method has the advantage of giving a specific PRP response.
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Medium components from the early part of the fermentation interfered with the analyses.