Determination of 20 coccidiostats in egg and avian muscle tissue using ultra high performance liquid chromatography-tandem mass spectrometry

详细信息    查看全文

• 作者：Mary Moloney ; Lesa Clarke ; John O¡¯Mahony ; Anna Gadaj ; Richard O¡¯Kennedy ; Martin Danaher
• 关键词：Coccidiostats ; Ionophores ; UHPLC&ndash ; MS/MS ; Egg ; Avian muscle
• 刊名：Journal of Chromatography A
• 出版年：2012
• 出版时间：31 August, 2012
• 年：2012
• 卷：1253
• 期：Complete
• 页码：94-104
• 全文大小：495 K

文摘

A quantitative, comprehensive multiresidue method which includes 20 coccidiostat residues has been developed. The method described uses a simple one-step liquid extraction with acetonitrile to isolate analytes from both the polyether ionophore and chemical classes of coccidiostats. Subsequent to a further concentration step, samples were analysed via UHPLC-MS/MS. The method was validated according to the Commission Decision 2002/657/EEC in egg and avian muscle. The method permitted quantitative confirmation for 13 compounds below target concentrations, and screening for a further 7 compounds. Within-laboratory repeatability gave accuracy values in the range of 68-129 % , while reproducibility ranged between 75 and 123 % . Calibration ranges were typically 1-50 ¦Ìg kg^?1, although higher ranges were used for dinitrocarbanilide, imidocarb and toltrazuril residues. A regression coefficient (R²) value of greater than 0.98 was obtained for all analytes. Precision results ranged from 2.3 to 19.7 % CV for egg and from 2.6 to 23.6 % CV in muscle. CC¦Á was in the range from 1.13 ¦Ìg kg^?1 (clopidol) to 179 ¦Ìg kg^?1 (lasalocid) in egg. In muscle, CC¦Á ranged from 2.25 ¦Ìg kg^?1 (aprinocid) to 4579 ¦Ìg kg^?1 (dinitrocarbanilide). CC¦Â was from 1.29 ¦Ìg kg^?1 (clopidol) to 209 ¦Ìg kg^?1 (lasalocid) in egg, and 2.58 ¦Ìg kg^?1 (arprinocid) to 6060 ¦Ìg kg^?1 (dinitrocarbanilide) in muscle. Limits of quantification were 1 ¦Ìg kg^?1 for all compounds, except imidocarb and dinitrocarbanilide (10 ¦Ìg kg^?1), and toltrazuril and metabolites (50 ¦Ìg kg^?1).