Design and implementation of an imprinted material for the extraction of the endocrine disruptor bisphenol A from milk

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• 作者：John O¡¯Mahony ; Mary Moloney ; Martin McCormack ; Ian A. Nicholls ; Boris Mizaikoff ; Martin Danaher
• 关键词：MIP ; Bisphenol A ; SPE ; Milk ; UHPLC&ndash ; MS/MS ; HPLC-FLD
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2013
• 出版时间：15 July, 2013
• 年：2013
• 卷：931
• 期：Complete
• 页码：164-169
• 全文大小：893 K

文摘

This paper describes the determination of bisphenol A (BPA) in milk samples, using a novel molecularly imprinted polymer. The imprinted polymer was developed using a rational design approach, and pre-polymerization interactions were investigated using molecular dynamics simulations and X-ray crystallography. A hydroquinone-imprinted polymer was used for solid phase extraction (SPE) clean-up of samples. BPA was quantified by high performance liquid chromatography (HPLC) and fluorescence (FLD) detection. Following validation, the method described was capable of determining bisphenol A in milk down to a limit of detection of 1.32 ¦Ìg kg^?1. The method was applied to a survey (n = 27) of commercial milk products; BPA was detected in one of the samples, at a level of 176 ¦Ìg kg^?1. Test results were confirmed by a parallel UHPLC-MS/MS analytical method. This demonstrates the utility of the hydroquinone-imprinted polymer for application to selective sample clean-up and analysis of bisphenol A in milk, avoiding possible detrimental affects associated with template bleeding and without the need for expensive or difficult-to-obtain template.