Antibody response and virus tissue distribution in chickens inoculated with wild-type and recombinant fowl adenoviruses

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• 作者：Ojkic ; Davor ; Nagy ; É ; va
• 关键词：Fowl adenovirus ; Gene expression vector ; Tandem repeats ; Immune response ; Virus distribution
• 刊名：Vaccine
• 出版年：2003
• 出版时间：December 8, 2003
• 年：2003
• 卷：22
• 期：1
• 页码：42-48
• 全文大小：109 K

文摘

We demonstrated that the long tandemly repeated region (TR-2) is dispensable for in vitro replication of fowl adenovirus 9 (FAdV-9). The TR-2-deleted recombinant FAdV-9 expressing the enhanced green fluorescence protein was further characterized for in vivo effects. Groups of chickens were exposed to recombinant or wild-type FAdV-9 by intramuscular injection, through the feed or drinking water and one group served as a negative control. The antibody (Ab) response, evaluated by ELISA and a plaque reduction test depended on the virus, dosage and the route of inoculation. Although the highest levels of anti-viral Ab were detected in chickens inoculated intramuscularly (i.m.) with wild-type FAdV-9, the deletion of TR-2 did not have a significant effect on the immune response. The tissue distribution of the virus was examined by the polymerase chain reaction (PCR) and was similar for both wild-type and recombinant viruses. Based on these results the TR-2 was dispensable for viral replication in vivo and did not influence virus distribution, and the recombinant FAdV-9 induced the same immune response as the wild-type virus.