- 作者：Hamdi H. Hamama ; BDS ; MDS ; PhD&lowast ; ; Cynthia K. Yiu ; BDS ; MDS ; PhD&dagger ; ; ckyyiu@hkucc.hku.hk" class="auth_mail" title="E-mail the corresponding author ; Michael F. Burrow ; BDS ; MDS ; DDSc ; PhD&Dagger ;
- 关键词：Carisolv ; chemomechanical caries removal ; confocal laser microscopy ; Papacarie ; Streptococcus mutans
- 刊名：Journal of Endodontics
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：40
- 期：12
- 页码：1972-1976
- 全文大小：899 K
Methods
Twenty-five caries-free dentin discs prepared from 25 sound maxillary premolars were used. The discs were then infected with Streptococcus mutans suspension and randomly divided into the following 6 groups according to the dentin treatments: the negative control group: noninfected sound dentin discs, the positive control group: infected discs were left untreated; the NaOCl group: treated with 5% NaOCl solution, the chlorhexidine (CHX) group: 2% CHX solution, the Carisolv group: Carisolv gel (Medi Team Dentalutveckling AB, Savedalen, Sweden), and the Papacarie group: Papacarie gel (Formula & Acao, São Paulo, Brazil). All the agents were applied for 5 minutes. The dentin discs were fractured into 2 halves and stained with fluorescent LIVE/DEAD Stain (Molecular Probes, Eugene, OR). Each specimen was observed using confocal laser scanning microscopy at 5 different randomly selected sites.
Results
The results of 1-way analysis of variance revealed that 5% NaOCl solution achieved the highest intratubular antibacterial effect, whereas Carisolv gel had the lowest antibacterial effect (P < .05). No significant difference in antibacterial effect was observed between the Papacarie gel and 2% CHX solution (P > .05).
Conclusions
The enzyme-based chemomechanical caries removal (Papacarie) was effective in the reduction of residual cariogenic bacteria in the dentinal tubules of coronal dentin.