Synergism of Dam, MutH, and MutS in methylation-directed mismatch repair in Escherichia coli

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• 作者：Changkun Hu^a ; ¹ ; Yunqi Zhao^a ; ^b ; ¹ ; Huiyun Sun^a ; Yixin Yang^a ; ^{yyang@emporia.edu}
• 关键词：DNA methylation-directed mismatch repair ; MutS ; MutH ; Dam
• 刊名：Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：795
• 期：Complete
• 页码：31-33
• 全文大小：390 K
• 卷排序：795

文摘

DNA mismatch repair (MMR) is a critical mutation surveillance system for recognizing and repairing erroneous insertion, deletion, and disincorporation of base. Major components of mismatch repair system consist of MutH, MutL, and MutS. Dam methylates adenine to distinguish newly synthesized daughter strands from the parent strands. Employing a tyrosine-auxotrophic E. coli FX-11 strain, the mutation frequency can be determined by the number of tyrosine revertants and the cell viability of FX-11 with deficiencies in dam and mismatch repair proteins. This study showed that mutS defect produced a higher mutation frequency than mutH did. Interestingly, double defects in dam and mutS synergistically produced a dramatically higher spontaneous mutation frequency than the summation of mutation frequencies of FX-11 strains with individual deficiency of dam or mutS, suggesting that Dam may work with MutHL to partially accomplish the task of recognizing the mismatch sites to retain partial mismatch repair capacity.