Immunosensing by luminescence reduction in surface-modified microstructured SU-8
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- 作者：Pinkie Jacob Eravuchiraa ; Malgorzata Baranowskaa ; Chris Ecksteina ; Francesc Dí ; azb ; Eduard Llobeta ; Lluis F. Marsala ; Josep Ferré ; -Borrulla ; josep.ferre@urv.cat" class="auth_mail" title="E-mail the corresponding author
- 关键词：MEMS ; micro-electro mechanical systems ; PL ; photoluminescence ; IgG ; immunoglobulin ; aIgG ; inunoglobulin antigen ; GTA ; glutaraldehyde ; PBS ; phosphate buffer saline ; PGMEA ; 1-methoxy-2-propanol acetate ; APTMS ; aminopropyltriethoxysilane ; DI H2O ; deionised water ; DMSO ; dimethyl sulfoxide ; FTIR ; Fourier-transform infrared spectroscopy ; ATR ; attenuated total reflection ; LOD ; limit of detection
- 刊名：Applied Surface Science
- 出版年：2017
- 出版时间：15 January 2017
- 年：2017
- 卷：392
- 期：Complete
- 页码：883-888
- 全文大小：1683 K
文摘
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The reduction of photoluminescence of SU-8 upon surface modification is reported.
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Micropillar structuring of SU-8 surface results in an increased photoluminescence reduction rate (10% glass, 15% silicon).
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Photoluminescence reduction rate can be a transduction parameter for the detection of antibody-antigen binding events.
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The proposed sensing mechanism can be used to quantify small concentrations of antibody.
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Lower limit of detection (LOD) of 28 μg/ml on silicon substrates and 42 μg/ml on glass substrates was achieved.