Studying the denaturation of bovine serum albumin by a novel approach of difference-UV analysis
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- 作者：Athanasios Nikolaidis ; Thomas Moschakis ; tmoschak@agro.auth.gr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Difference-UV spectroscopy ; BSA ; Heat denaturation ; GdHCl ; Activation energy ; DSC
- 刊名：Food Chemistry
- 出版年：2017
- 出版时间：15 January 2017
- 年：2017
- 卷：215
- 期：Complete
- 页码：235-244
- 全文大小：1720 K
文摘
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Novel approach to difference-UV spectra analysis.
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Peak integration and peak height as method to determine protein denaturation.
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BSA exhibits structured conformation even after heating at long times.
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“Breaks” in the Arrhenius plots at around 67 °C.
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Activation Energy calculated at around 135 kJ/mol.