How the imidazole ring modulates amyloid formation of islet amyloid polypeptide: A chemical modification study
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- 作者：Xin Zhanga ; 1 ; Junjun Liua ; 1 ; lianqi Huanga ; Xin Yanga ; Robert B. Petersenb ; c ; d ; Yue Sune ; Hao Gonga ; Ling Zhenge ; lzheng217@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Kun Huanga ; kunhuang2008@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：IAPP ; islet amyloid polypeptide ; hIAPP ; human IAPP ; DEPC ; diethylpyrocarbonate ; DMI ; DEPC-modified hIAPP ; HFIP ; 1 ; 1 ; 1 ; 3 ; 3 ; 3-hexafluoro-2-propanol ; ThT ; thioflavin-T
- 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：1860
- 期：4
- 页码：719-726
- 全文大小：1181 K
文摘
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We use DEPC to specifically modify and obtain mono-ethyloxyformylated hIAPP (DMI).
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After DEPC modification, the pH dependent hIAPP fibrillation went to opposite order.
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DEPC modification decreases the amount intra-molecular hydrogen bonds formed.
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DEPC modification impairs the membrane–peptide interaction.
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DEPC modification impairs the ion–peptide interaction.