- 作者：Jing Qiana ; b ; qianjing198722@126.com" class="auth_mail" title="E-mail the corresponding author ; Chunhua Rena ; c ; rosemary166@sina.com" class="auth_mail" title="E-mail the corresponding author ; Jianjun Xiaa ; jianjunxia@scsio.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Ting Chena ; c ; chan1010@scsio.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Zonghe Yua ; yuzh@scsio.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Chaoqun Hua ; c ; cqhu@scsio.ac.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：A2M ; alpha-2-macroglobulin ; BRD ; bait region domain ; TED ; thioester domain ; RBD ; receptor-binding domain ; T-cell ; thymus-derived cell ; proPO ; prophenoloxidase ; H. atra ; Holothuria atra ; cDNA ; DNA complementary to RNA ; mRNA ; messenger RNA ; Poly (I ; C) ; polyriboinosinic polyribocytidylic acid ; RNAi ; RNA interference ; DEPC ; Diethylpyrocarbonate ; RNA ; ribonucleic acid ; V. alginolyticus ; Vibrio alginolyticus ; oligo ; oligodeoxyribonucleotide ; PCR ; polymerase chain reaction ; bp ; base pair(s) ; RACE ; rap
- 刊名：Gene
- 出版年：2016
- 出版时间：10 July 2016
- 年：2016
- 卷：585
- 期：2
- 页码：205-215
- 全文大小：2336 K
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The full-length cDNA of alpha-2-macroglobulin (A2M) was obtained from the sea cucumber Holothuria atra.
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Higher expression level of HaA2M was found in the body wall and coelomocytes, which are involved in body wall and cellular response, respectively.
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The expression of HaA2M in immune tissues by treatment with V. alginolyticus and Poly (I: C) indicated that HaA2M is an immune-related gene.
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The changes in HaA2M activity by treatment with V. alginolyticus and Poly (I: C) indicated that A2M is an immune-related protein.
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In this study, putative differences in the HaA2M mRNA and A2M activity levels were investigated in H. atra.
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The gene silencing of HaA2M suggested that HaA2M is an essential molecule in the innate defense against V. alginolyticus infections.