Determination of human reference values for serum total 1,25-dihydroxyvitamin D using an extensively validated 2D ID-UPLC-MS/MS method
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- 作者：Niek F. Dirksa ; Frans Martensa ; Dirk Vanderschuerenb ; c ; Jaak Billenb ; c ; Steven Pauwelsc ; d ; Marië ; tte T. Ackermanse ; Erik Enderte ; Martin den Heijerf ; Marinus A. Blankensteina ; Annemieke C. Heijboera ; a.heijboer@vumc.nl" class="auth_mail" title="E-mail the corresponding author
- 关键词：DEQAS ; vitamin D External Quality Assessment Scheme ; 25OHD ; 25-hydroxyvitamin D ; 24 ; 25diOHD ; 24 ; 25-dihydroxyvitamin D ; 1 ; 25diOHD ; 1 ; 25-dihydroxyvitamin D ; VDR ; vitamin D receptor ; RIA ; radioimmunoassay ; 2D ID-UPLC&ndash ; MS/MS ; two-dimensional isotope dilution ultra pressure liquid chromatography&ndash ; tandem mass spectrometry ; FA ; formic acid ; BSA ; bovine serum albumin ; PBS ; phosphate-buffered saline ; PTAD ; 4-phenyl-1 ; 2 ; 4-triazole-3 ; 5-dione ; CV ; coefficient of variation ; LOQ ; limit of quantitati
- 刊名：Journal of Steroid Biochemistry and Molecular Biology
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：164
- 期：Complete
- 页码：127-133
- 全文大小：406 K
文摘
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We have developed a LC–MS/MS method able to measure both 1,25dihydroxyvitamin D3 and D2.
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The method was extensively validated with regard to sensitivity, specificity and robustness and correlated well with another published LC–MS/MS method.
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Human reference values for 1,25-dihydroxyvitamin D3 and D2 were 59–159 pmol/L and <17 pmol/L, respectively.
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Premenopausal women taking oral contraceptive pills show significantly higher 1,25-dihydroxyvitamin D3 values compared to postmenopausal women.