Development and validation of an LC-MS/MS based method for quantification of 25 hydroxyvitamin D2 and 25 hydroxyvitamin D3 in human serum and plasma
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- 作者：Stanley (Weihua) Zhanga ; wzhang7@its.jnj.com" class="auth_mail ; Wenying Jianb ; Sheryl Sullivana ; Banu Sankarana ; Richard W. Edomb ; Naidong Wengb ; David Sharkeya
- 关键词：25(OH)D ; 25-hydroxyvitamin D ; 25(OH)D2 ; 25-hydroxyvitamin D2 ; 25(OH)D3 ; 25-hydroxyvitamin D3 ; 1 ; 25(OH)2D ; 1 ; 25-dihydroxyvitamin D ; DBP ; vitamin D binding protein ; RIA ; radioimmunoassay ; ELISA ; enzyme-linked immunosorbent assay ; HPLC ; high performance liquid chromatography ; LC&ndash ; MS/MS ; liquid chromatography&ndash ; tandem mass spectrometry ; DEQAS ; the Vitamin D External Quality Assessment Scheme ; QC ; Quality control ; MRM ; Multiple reaction monitoring ; BSA ; Bovine serum albumin ; PBS ; phosphate-b
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：15 June 2014
- 年：2014
- 卷：961
- 期：Complete
- 页码：62-70
- 全文大小：1133 K
文摘
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An LC–MS/MS based method for 25(OH)D2 and 25(OH)D3 quantification was validated.
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A complete stability profile of standard solutions and QC samples was obtained.
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No differences were found between matching serum, heparin, and EDTA plasma samples.
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25(OH)D concentrations measured were close to the DEQAS LC–MS/MS Method Means.
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The LC–MS/MS method showed limited correlation to DiaSorin Liaison 25(OH)D assay.