Involvement of intracellular cAMP in epirubicin-induced vascular endothelial cell injury

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• 作者：Takaaki Yamada^a ; Mitsuyo Ueda^b ; Nobuaki Egashira^a ; ^b ; ^{n-egashi@pharm.med.kyushu-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Nina Zukeyama^b ; Jun Kuwahara^b ; Satohiro Masuda^a ; ^b
• 关键词：Epirubicin ; Endothelial cell ; cAMP
• 刊名：Journal of Pharmacological Sciences
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：130
• 期：1
• 页码：33-37
• 全文大小：568 K

文摘

We investigated the involvement of intracellular cAMP in endothelial cell injury induced by epirubicin. Epirubicin-induced decrease in cell viability and increase in caspase-3/7 activity were reversed by a cAMP analog dibutyryl cAMP (DBcAMP) or an activator of adenylate cyclase forskolin concomitant with a phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine. Moreover, epirubicin-induced elevation of lipid peroxide levels was attenuated by DBcAMP. Interestingly, the exposure of epirubicin decreased intracellular cAMP levels before the onset of epirubicin-induced production of lipid peroxidation. These results suggest that intracellular cAMP plays an important role in epirubicin-induced endothelial cell injury.