Testing the absorption of the extracts of Coreopsis tinctoria Nutt. in the intestinal canal in rats using an Ussing chamber

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• 作者：Jian Wang^a ; Guzhalinuer Aierken^b ; Xinxia Li^b ; Linlin Li^a ; Xinmin Mao^a ; ^c ; ^{mxm3277@sina.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Coreopsis tinctoria Nutt. ; Ussing chamber model ; HPLC ; QAMS ; Absorption characteristics
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：20 June 2016
• 年：2016
• 卷：186
• 期：Complete
• 页码：73-83
• 全文大小：2162 K

文摘

Coreopsis tinctoria Nutt mainly distributed in Hetian region of Xinjiang at an altitude of 3000 m, which is used as Uyghur traditional medicine because of its clearing heat, promoting circulation and removing toxicity and antihypertension, ect. effect.

Aim of the study

5">This research was to study the four ingredients in the extracts of Coreopsis tinctoria Nutt. that are absorbed in different intestinal segments in rats to lay the foundation for further study on the effective constituents, tissue distribution, metabolism, and spectrum-effect relationships of these extracts.

Materials and methods

High, medium, and low concentrations were prepared according to their pharmacological effects. Quantitative analysis multi-components by single marker was used to test the cumulative absorption volume Q, absorption rate constant Ka, and apparent permeability coefficient P_app of the four main ingredients in C. tinctoria Nutt. extract in different intestinal segments in rats using a Ussing chamber model and high-performance liquid chromatography.

Results

5">The P_app of chlorogenic acid and flavanomarein in the duodenum, jejunum, ileum, and colon were 1.0×10⁻⁶ to 10×10⁻⁶ cm s⁻¹. P_app of marein in the duodenum and jejunum was <1.0×10⁻⁶, and was 1.0×10⁻⁶ to 10×10⁻⁶ cm s⁻¹ in the ileum and colon. P_app of 3,5-O-dicaffeoylquinic acid in the duodenum was <1.0×10⁻⁶ cm s⁻¹, while it was 1.0×¹0⁻⁶ to 10×10⁻⁶ cm s⁻¹ in the jejunum, ileum, and colon.

5">Conclusions

All four chemical components of the plant extract can be absorbed by the intestinal canal of rats, which conforms to zero-order absorption; the ileum presented the best absorption.