Collagen fibers mediate MRI-detected water diffusion and anisotropy in breast cancers

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• 作者：Samata Kakkad^* ; ^&Dagger ; ; Jiangyang Zhang^* ; Alireza Akhbardeh^* ; Desmond Jacob^* ; Balaji Krishnamachary^* ; Meiyappan Solaiyappan^* ; Michael A. Jacobs^* ; ^&dagger ; ; Venu Raman^* ; ^&dagger ; ; Dieter Leibfritz^&Dagger ; ; Kristine Glunde^* ; ^&dagger ; ; Zaver M. Bhujwalla^* ; ^&dagger ; ; ^{zaver@mri.jhu.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：2D ; two-dimensional ; 3D ; three-dimensional ; aDW ; average diffusion weighted ; ADC ; apparent diffusion coefficient ; Col1 ; collagen 1 ; DTI ; diffusion tensor imaging ; ECM ; extracellular matrix ; ER ; estrogen receptor ; FA ; fractional anisotropy ; FOV ; field of view ; H&E ; hematoxylin and eosin ; HER-2 ; human epidermal growth factor receptor 2 ; HIF-1 ; hypoxia inducible factor ; HRE ; hypoxia response element ; IDC ; invasive ductal carcinoma ; LN ; lymph node ; MRI ; magnetic resonance imaging ; PFA ; paraformaldeh
• 刊名：Neoplasia
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：18
• 期：10
• 页码：585-593
• 全文大小：1713 K

文摘

Collagen 1 (Col1) fibers play an important role in tumor interstitial macromolecular transport and cancer cell dissemination. Our goal was to understand the influence of Col1 fibers on water diffusion, and to examine the potential of using noninvasive diffusion tensor imaging (DTI) to indirectly detect Col1 fibers in breast lesions. We previously observed, in human MDA-MB-231 breast cancer xenografts engineered to fluoresce under hypoxia, relatively low amounts of Col1 fibers in fluorescent hypoxic regions. These xenograft tumors together with human breast cancer samples were used here to investigate the relationship between Col1 fibers, water diffusion and anisotropy, and hypoxia. Hypoxic low Col1 fiber containing regions showed decreased apparent diffusion coefficient (ADC) and fractional anisotropy (FA) compared to normoxic high Col1 fiber containing regions. Necrotic high Col1 fiber containing regions showed increased ADC with decreased FA values compared to normoxic viable high Col1 fiber regions that had increased ADC with increased FA values. A good agreement of ADC and FA patterns was observed between in vivo and ex vivo images. In human breast cancer specimens, ADC and FA decreased in low Col1 containing regions. Our data suggest that a decrease in ADC and FA values observed within a lesion could predict hypoxia, and a pattern of high ADC with low FA values could predict necrosis. Collectively the data identify the role of Col1 fibers in directed water movement and support expanding the evaluation of DTI parameters as surrogates for Col1 fiber patterns associated with specific tumor microenvironments as companion diagnostics and for staging.