Comparison of the quantification of KRAS mutations by digital PCR and E-ice-COLD-PCR in circulating-cell-free DNA from metastatic colorectal cancer patients

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• 作者：David Sefrioui^a ; ^b ; ¹ ; Florence Mauger^c ; ¹ ; Laurence Leclere^c ; Ludivine Beaussire^b ; Fré ; dé ; ric Di Fiore^a ; ^b ; Jean-Franç ; ois Deleuze^c ; Nasrin Sarafan-Vasseur^b ; Jö ; rg Tost^c ; ^tost@cng.fr
• 关键词：dPCR ; digital PCR ; ddPCR ; digital droplet PCR ; E-ice-COLD-PCR ; Enhanced-improved and complete-coamplification at lower denaturation temperature PCR ; ccfDNA ; circulating cell-free DNA
• 刊名：Clinica Chimica Acta
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：465
• 期：Complete
• 页码：1-4
• 全文大小：460 K
• 卷排序：465

文摘

Both digital PCR and E-ice-COLD-PCR assays allow the sensitive detection of mutations in cell-free circulating DNA. Quantitative precision down to clinically important thresholds is similar. E-ice-COLD-PCR assays use standard laboratory equipment and have a shorter time to results. E-ice-COLD-PCR detects, identifies and quantitates mutations without prior knowledge on the location and nucleotide change. Digital PCR and E-ice-COLD-PCR assays allow monitoring mutations during personalized management of cancer patients.