Comparison of the quantification of KRAS mutations by digital PCR and E-ice-COLD-PCR in circulating-cell-free DNA from metastatic colorectal cancer patients
Both digital PCR and E-ice-COLD-PCR assays allow the sensitive detection of mutations in cell-free circulating DNA. Quantitative precision down to clinically important thresholds is similar. E-ice-COLD-PCR assays use standard laboratory equipment and have a shorter time to results. E-ice-COLD-PCR detects, identifies and quantitates mutations without prior knowledge on the location and nucleotide change. Digital PCR and E-ice-COLD-PCR assays allow monitoring mutations during personalized management of cancer patients.