Crotalaria juncea oil is trans-esterified, waste glycerol is separated and purified.
Crude, purified and commercial glycerol becomes the major carbon source for E.coli.
Succinic acid is produced by batch fermentation of glycerol using Escherichia coli.
Aiba-Edward model ranked 1 out of 7 in case of two samples.
Process parameters for the batch fermentation are optimised.