Knockdown of a putative insulin-like peptide gene LdILP2 in Leptinotarsa decemlineata by RNA interference impairs pupation and adult emergence
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- 作者：Kai-Yun Fua ; 176061610@qq.com" class="auth_mail" title="E-mail the corresponding author ; Tao-Tao Zhua ; 475652324@qq.com" class="auth_mail" title="E-mail the corresponding author ; Wen-Chao Guob ; gwc1966@163.com" class="auth_mail" title="E-mail the corresponding author ; Tursun Ahmatb ; Tu1015@163.com" class="auth_mail" title="E-mail the corresponding author ; Guo-Qing Lia ; ligq@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：JH ; juvenile hormone ; JHAMT ; juvenile hormone acid methyltransferase ; 20E ; 20-hydroxyecdysone ; IIS ; insulin/insulin-like growth factor signaling pathway ; RNAi ; RNA interference ; dsRNA ; double-stranded RNA ; ANOVA ; analysis of variance ; CYP ; cytochrome P450 monooxygenase ; PG ; prothoracic glands ; CA ; corpora allata
- 刊名：Gene
- 出版年：2016
- 出版时间：1 May 2016
- 年：2016
- 卷：581
- 期：2
- 页码：170-177
- 全文大小：1426 K
文摘
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Five insulin-like peptide genes were cloned.
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RNAi of LdILP2 impaired pupation and adult emergence.
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RNAi of LdILP2 inhibited IIS signaling pathway.
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JH and 20E titers were lowered in the LdILP2 RNAi larvae.
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JH and 20E pathways were disrupted in the LdILP2 RNAi larvae.