Dietary supplementation of aspartate enhances intestinal integrity and energy status in weanling piglets after lipopolysaccharide challenge

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• 作者：Dingan Pi^a ; Yulan Liu^a ; ^{yulanflower@126.com" class="auth_mail} ; Haifeng Shi^a ; Shuang Li^a ; Jack Odle^b ; Xi Lin^b ; Huiling Zhu^a ; Feng Chen^a ; Yongqing Hou^a ; Weibo Leng^a
• 关键词：Aspartate ; Intestine ; Energy status ; Weanling piglets ; Lipopolysaccharide
• 刊名：Journal of Nutritional Biochemistry
• 出版年：April, 2014
• 年：2014
• 卷：25
• 期：4
• 页码：456-462
• 全文大小：281 K

文摘

The intestine has a high requirement for ATP to support its integrity, function and health, and thus, energy deficits in the intestinal mucosa may play a critical role in intestinal injury. Aspartate (Asp) is one of the major sources of ATP in mammalian enterocytes via mitochondrial oxidation. We hypothesized that dietary supplementation of Asp could attenuate lipopolysaccharide (LPS)-induced intestinal damage via modulation of intestinal energy status. Twenty-four weanling piglets were allotted to one of four treatments: (1) nonchallenged control, (2) LPS-challenged control, (3) LPS+0.5% Asp treatment, and (4) LPS+1.0% Asp treatment. On day 19, pigs were injected with saline or LPS. At 24 h postinjection, pigs were killed and intestinal samples were obtained. Asp attenuated LPS-induced intestinal damage indicated by greater villus height and villus height/crypt depth ratio as well as higher RNA/DNA and protein/DNA ratios. Asp improved intestinal function indicated by increased intestinal mucosal disaccharidase activities. Asp also improved intestinal energy status indicated by increased ATP, ADP and total adenine nucleotide contents, adenylate energy charge and decreased AMP/ATP ratio. In addition, Asp increased the activities of tricarboxylic acid cycle key enzymes including citrate synthase, isocitrate dehydrogenase and alpha-oxoglutarate dehydrogenase complex. Moreover, Asp down-regulated mRNA expression of intestinal AMP-activated protein kinase 伪1 (AMPK伪1), AMPK伪2, silent information regulator 1 (SIRT1) and peroxisome proliferator-activated receptor gamma coactivator-1伪 (PGC1伪) and decreased intestinal AMPK伪 phosphorylation. These results indicate that Asp may alleviate LPS-induced intestinal damage and improve intestinal energy status.