Two Leptinotarsa uridine diphosphate N-acetylglucosamine pyrophosphorylases are specialized for chitin synthesis in larval epidermal cuticle and midgut peritrophic matrix
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- 作者：Ji-Feng Shia ; shijf1214@126.com" class="auth_mail" title="E-mail the corresponding author ; Jia Fua ; fujia878@163.com" class="auth_mail" title="E-mail the corresponding author ; Li-Li Mua ; mll700819@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Wen-Chao Guob ; gwc1966@163.com" class="auth_mail" title="E-mail the corresponding author ; Guo-Qing Lia ; ligq@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Uridine diphosphate-N-acetylglucosamine-pyrophosphorylase ; Chitin ; Ecdysis ; Hormone
- 刊名：Insect Biochemistry and Molecular Biology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：68
- 期：Complete
- 页码：1-12
- 全文大小：3967 K
文摘
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Two UDP N-acetylglucosamine pyrophosphorylase genes were cloned from the potato beetle, Leptinotarsa decemlineata.
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Expression of LdUAP1 and LdUAP2 responded to 20-hydroxyecdysone and juvenile hormone.
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RNAi knockdown of LdUAP1 reduced chitin contents and impaired ecdysis.
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LdUAP2 RNAi damaged the peritrophic matrix and blocked development.