Antifungal depsidone metabolites from Cordyceps dipterigena, an endophytic fungus antagonistic to the phytopathogen Gibberella fujikuroi

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• 作者：Titto Varughese^a ; ^b ; Nivia Rios^a ; Sarah Higginbotham^b ; A. Elizabeth Arnold^c ; Phyllis D. Coley^b ; ^d ; Thomas A. Kursar^b ; ^d ; William H. Gerwick^e ; Luis Cubilla Rios^a ; ^b ; ^{lucr@ancon.up.pa} ; ^{lucr@ancon.up.ac}
• 关键词：Fungal metabolite ; Cordycepsidone ; Cordyceps dipterigena ; Gibberella fujikuroi ; Antifungal activity
• 刊名：Tetrahedron Letters
• 出版年：2012
• 出版时间：28 March, 2012
• 年：2012
• 卷：53
• 期：13
• 页码：1624-1626
• 全文大小：175 K

文摘

Among thirty four endophytic fungal strains screened for in vitro antagonism, the endophytic fungus Cordyceps dipterigena was found to strongly inhibit mycelial growth of the plant pathogenic fungus Gibberella fujikuroi. Two new depsidone metabolites, cordycepsidone A (1) and cordycepsidone B (2), were isolated from the PDA culture extract of C. dipterigena and identified as being responsible for the antifungal activity. Elucidation of their chemical structures was carried out using 1D and 2D NMR spectroscopy in combination with IR and MS spectroscopic data. Cordycepsidone A displayed strong and dose-dependent antifungal activity against the plant pathogenic fungus G. fujikuroi. The isolates were inactive in bioassays for malaria (Plasmodium falciparum), leishmaniasis (Leishmania donovani), Chagas¡®s disease (Trypanosoma cruzi), and cytotoxicity at 10 ¦Ìg/mL. The compounds were also found to be inactive against several bacterial strains at 50 ¦Ìg/mL.