Disease control efficacy of synthetic double-stranded RNA Poly(I:C) administration for viral nervous necrosis (VNN) in sevenband grouper (Epinephelus septemfasciatus)
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- 作者：W.-S. Kim ; C.-S. Kim ; J.-K. Cho ; J.-I. Myung ; M.-J. Oh
- 关键词：Poly(I ; C) ; Viral nervous necrosis ; Sevenband grouper ; Disease control
- 刊名：Aquaculture
- 出版年：2012
- 出版时间：5 October, 2012
- 年：2012
- 卷：364-365
- 期：Complete
- 页码：259-262
- 全文大小：358 K
文摘
Nervous necrosis virus (NNV), the causative agent of viral nervous necrosis (VNN), is an indigenous virus in Korean coastal areas and a threat to the farming industry. In the present study, we investigated the curative and preventive effects against VNN in sevenband grouper (Epinephelus septemfasciatus) using polyinosinic polycytidylic acid [Poly(I:C)]. Fish challenged with brain-filtrates from VNN-affected fish at 0, 2, and 4 d after Poly(I:C) injection showed < 10 % cumulative mortality, whereas fish that were not administered Poly(I:C) but challenged 14 d after the Poly(I:C) injection showed 100 % and 80 % cumulative mortality, respectively. The surviving fish from the primary challenge with brain-filtrates following Poly(I:C) injection were highly protected from re-challenge at 15 and 30 d post-primary challenge. Moreover, fish pre-immersed with red-spotted grouper nervous necrosis virus (RGNNV) survived following Poly(I:C) administration, even though their clinical signs did not recover. From these results, it was confirmed that sevenband grouper were highly protected from RGNNV challenge by Poly(I:C) administration.