Appropriate choice of collision-induced dissociation energy for qualitative analysis of notoginsenosides based on liquid chromatography hybrid ion trap time-of-flight mass spectrometry

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• 作者：Guang-Ji WANG^Δ ; Han-Xu FU^Δ ; Jing-Cheng XIAO ; Wei YE ; Tai RAO ; Yu-Hao SHAO ; Dian KANG ; Lin XIE ; Yan LIANG ; ^{liangyan0679@hotmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Collision energy ; Collision-induced dissociation ; Notoginsenosides ; Qualitative analysis
• 刊名：Chinese Journal of Natural Medicines
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：14
• 期：4
• 页码：278-285
• 全文大小：1517 K

文摘

Liquid chromatography hybrid ion trap/time-of-flight mass spectrometry possessesd both the MSⁿ ability of ion trap and the excellent resolution of a time-of-flight, and has been widely used to identify drug metabolites and determine trace multi-components for in natural products. Collision energy, one of the most important factors in acquiring MSⁿ information, could be set freely in the range of 10%–400%. Herein, notoginsenosides were chosen as model compounds to build a novel methodology for the collision energy optimization. Firstly, the fragmental patterns of the representatives for the authentic standards of protopanaxadiol-type and protopanaxatriol-type notoginsenosides authentic standards were obtained based on accurate MS² and MS³ measurements via liquid chromatography hybrid ion trap/time-of-flight mass spectrometry. Then the extracted ion chromatograms of characteristic product ions of notoginsenosides in Panax Notoginseng Extract, which were produced under a series of collision energies and, were compared to screen out the optimum collision energies values for MS² and MS³. The results demonstrated that the qualitative capability of liquid chromatography hybrid ion trap/time-of-flight mass spectrometry was greatly influenced by collision energies, and 50% of MS² collision energy was found to produce the highest collision-induced dissociation efficiency for notoginsenosides. BesidesAddtionally, the highest collision-induced dissociation efficiency appeared when the collision energy was set at 75% in the MS³ stage.