Combined use of peptide ion and normalized delta scores to evaluate milk authenticity by ion-trap based proteomics coupled with error tolerant searching
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- 作者：Donatella Nardiello ; donatella.nardiello@unifg.it ; Anna Natale ; Carmen Palermo ; Maurizio Quinto ; Diego Centonze
- 关键词：Normalized delta score ; Peptide ion score ; Ion-trap ; Enzymatic digestion ; Collision-Induced Dissociation (CID) ; Milk authenticity
- 刊名：Talanta
- 出版年：2017
- 出版时间：1 March 2017
- 年：2017
- 卷：164
- 期：Complete
- 页码：684-692
- 全文大小：516 K
- 卷排序：164
文摘
Database post-processing validation of MS/MS data sets in ion-trap proteomics. Combined peptide ion and normalized delta scores for confident sequence assignments. Error tolerant searching to maximize sequence coverage. Milk authenticity by identification of species-specific peptides. Direct analysis of tryptic/chymotryptic milk digests, without 2D-PAGE.