Quantification of the 3α and 3β epimers of 25-hydroxyvitamin D₃ in dried blood spots by LC-MS/MS using artificial whole blood calibration and chemical derivatization

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• 作者：Miriam J. Mü ; ller^a ; Caroline S. Stokes^b ; Dietrich A. Volmer^a ; ^{Dietrich.Volmer@mx.uni-saarland.de}
• 关键词：25(OH)D ; 25-hydroxyvitamin D ; ANOVA ; analysis of variance ; CID ; collision-induced dissociation ; DBS ; dried blood spots ; ESI ; electrospray ionization ; LC-MS/MS ; liquid chromatography-tandem mass spectrometry ; LOQ ; limit of quantification ; MRM ; multiple reaction monitoring ; PFP ; pentafluorophenyl ; UHPLC ; ultra high performance liquid chromatography
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 April 2017
• 年：2017
• 卷：165
• 期：Complete
• 页码：398-404
• 全文大小：389 K
• 卷排序：165

文摘

3α- and 3β-25-hydroxyvitamin D3 epimers were quantified from dried blood spots using LC-MS/MS. The assay was based on newly developed artificial vitamin D-free whole blood matrix. The calibration routine improved analytical performance over existing techniques. The method was compared across 3 different assay platforms with serum and whole blood.