Capillary electrophoresis coupled with 1,1鈥?thiocarbonyldiimidazole derivatization for the rapid detection of total homocysteine and cysteine in human plasma
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- 作者：Alexander Vladimirovich Ivanov ; Ivanov_av82@mail.ru" class="auth_mail" title="E-mail the corresponding author ; Edward Danielevich Virus ; Boris Petrovich Luzyanin ; Aslan Amirkhanovich Kubatiev
- 关键词：BGE ; background electrolyte ; CE ; capillary electrophoresis ; CTAB ; hexadecyltrimethylammonium bromide ; Cys ; cysteine ; CysGly ; cysteinylglycine ; CZE ; capillary zone electrophoresis ; DTNB ; 5 ; 5&prime ; -dithiobis(2-nitrobenzoic acid) ; DTT ; dithiotreitol ; EI ; electrokinetic injection ; ELISA ; enzyme-linked immunosorbent assay ; EDTA ; ethylenediaminetetraacetic acid ; Hcy ; homocysteine ; HPLC ; high performance liquid chromatography ; LOD ; limit of detection ; LOQ ; limit of quantification ; MS ; mass spectrometr
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2015
- 出版时间：1 November 2015
- 年：2015
- 卷：1004
- 期：Complete
- 页码：30-36
- 全文大小：853 K
文摘
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A new CE–UV method for quantification of homocysteine, cysteine and their ratio in plasma was validated.
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The time of analysis was 6 min.
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Specific derivatization of aminothioles using 1,1′-thiocarbonyldiimidazole.
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The method could be used for clinical researches.