10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy-2′-C-methyluridine in the catalytic core

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• 作者：Laura Robaldo ; Javier M. Montserrat ; Adolfo M. Iribarren
• 关键词：10-23 DNAzyme ; 2′ ; -Deoxy-2′ ; -C-methyl nucleosides
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2010
• 出版时间：1 August 2010
• 年：2010
• 卷：20
• 期：15
• 页码：4367-4370
• 全文大小：852 K

文摘

The catalytic core of a 10-23 DNAzyme was modified using (2′R), (2′S)-2′-deoxy-2′-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg²⁺ concentrations, indicating that certain 2′-C-methyl modified DNAzymes have significant activities. Resistance against MCF-7 cell lysate and endonuclease RQ1 was also measured, showing that the introduction of 2′-C-methyl-2′-deoxynucleosides increased the stability.