DNAzyme based gap-LCR detection of single-nucleotide polymorphism

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• 作者：Li Zhou ; Feng Du ; Yongyun Zhao ; Afshan Yameen ; Haodong Chen ; Zhuo Tang ; ^{tangzhuo@cib.ac.cn}
• 关键词：Gap-LCR ; DNAzyme ; Single-nucleotide polymorphism ; Exonuclease ; GJB2 235delC
• 刊名：Biosensors and Bioelectronics
• 出版年：2013
• 出版时间：15 July, 2013
• 年：2013
• 卷：45
• 期：Complete
• 页码：141-147
• 全文大小：708 K

文摘

Fast and accurate detection of single-nucleotide polymorphism (SNP) is thought more and more important for understanding of human physiology and elucidating the molecular based diseases. A great deal of effort has been devoted to developing accurate, rapid, and cost-effective technologies for SNP analysis. However most of those methods developed to date incorporate complicated probe labeling and depend on advanced equipment. The DNAzyme based Gap-LCR detection method averts any chemical modification on probes and circumvents those problems by incorporating a short functional DNA sequence into one of LCR primers. Two kinds of exonuclease are utilized in our strategy to digest all the unreacted probes and release the DNAzymes embedded in the LCR product. The DNAzyme applied in our method is a versatile tool to report the result of SNP detection in colorimetric or fluorometric ways for different detection purposes.