Rationale for enzymatic concentration of n-3 PUFAs into glycerides is defined.
Non-regiospecificity & high non-n-3 PUFAs preference are ideal catalytic feature.
CAL-A identified as a near-ideal enzyme for concentrating n-3 PUFAs via ethanolysis.
CAL-A affords concentration efficacy of n-3 PUFAs from 25% in oil to 90% in MAGs.
13CNMR analysis verifies the conceptual hypothesis and reveals catalytic mechanism.