Trafficking phenotype and production of granzyme B by double negative B cells (IgG+IgD鈭?/sup>CD27鈭?/sup>) in the elderly
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- 作者：Matteo Bulati ; Silvio Buffa ; Adriana Martorana ; Giuseppina Candore ; Domenico Lio ; Calogero Caruso ; Giuseppina Colonna-Romano ; giuseppina.colonnaromano@unipa.it" class="auth_mail
- 关键词：B lymphocytes ; Chemokine receptors ; Inflamm-aging ; Elderly ; IL-21 ; Granzyme B
- 刊名：Experimental Gerontology
- 出版年：June, 2014
- 年：2014
- 卷：54
- 期：Complete
- 页码：123-129
- 全文大小：514 K
文摘
The impairment of humoral immune response in elderly humans has been extensively demonstrated. We have reported the increase of memory B cells (IgG+IgD鈭?/sup>CD27鈭?/sup>, double negative, DN) population in the elderly, in which there is also a typical inflammatory micro-environment. In order to evaluate whether this pro-inflammatory status could influence the trafficking phenotype of na茂ve/memory B cells, we have assessed the expression of CCR7, CCR6, CXCR3, CXCR4, CXCR5 and CD62L on na茂ve/memory B cell subpopulations in young and elderly subjects. Moreover, the combination of pro-inflammatory interleukin-21 (IL-21) and B cell receptor (BCR) stimulation enables B cells to produce and secrete granzyme B (GrB), which plays a critical role in early anti-viral immune responses, in the regulation of autoimmune mechanisms and in cancer immunosurveillance.
Our data demonstrate that in the elderly, na茂ve/memory B cell populations present a different expression of the studied receptors that could be discussed in terms of 鈥渋nflamm-aging鈥? In particular IgG+IgD鈭?/sup>CD27鈭?/sup> DN B cells show a tissue trafficking phenotype and they can be stimulated to produce GrB.