The suppression of zfpm-1 accelerates the erythropoietic differentiation of human CD34⁺ cells

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• 作者：Hee-Young Yang ; Sue Hee Kim ; Seok-Ho Kim ; Dong-Jun Kim ; Sun-Uk Kim ; Dae-Yeul Yu ; Young Il Yeom ; Dong-Seok Lee ; Yong-Jun Kim ; Byung-Ju Park ; et al.
• 关键词：Hematopoietic stem cell ; Erythropoiesis ; Zfpm-1 ; GATA-1 ; c-myc ; c-myb ; Zfpm-2
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2007
• 出版时间：23 February 2007
• 年：2007
• 卷：353
• 期：4
• 页码：978-984
• 全文大小：469 K

文摘

Erythropoiesis is a complex multistage process for the differentiation of mature erythrocytes from hematopoietic stem cells. The function of several transcription factors has been reported in hematopoietic stem cell differentiation. However, the molecular basis governing its functional behavior is unclear. In this study, we characterized the role of Zfpm-1 during the erythropoietic differentiation of human hematopoietic stem cells. To verify the function of Zfpm-1 during erythropoietic differentiation, we established human CD34⁺ cell culture system by using human umbilical cord blood. At day 7 of the human CD34⁺ cell differentiation process to proerythocytes, Zfpm-1 was initially up-regulated and then dramatically down-regulated at day 9. The Zfpm-1 siRNA transfected HSCs contained 20 % more GPA⁺ cells than the mock transfected cells, and showed repressed expression of the hematopoietic transcription factors, c-myc and c-myb, but increased expression of GATA-1. In contrast, the Zfpm-1 gain-of-function is the opposite of loss-of-function results above.