Real-time Near-infrared Virtual Intraoperative Surgical Photoacoustic Microscopy

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• 作者：Changho Lee^a ; ^b ; ¹Author Vitae ; Donghyun Lee^b ; ¹Author Vitae ; Qifa Zhou^cAuthor Vitae ; Jeehyun Kim^dAuthor Vitae ; Chulhong Kim^a ; ^b ; ^{chulhong@postech.edu" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 刊名：Photoacoustics
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：3
• 期：3
• 页码：100-106
• 全文大小：2132 K

文摘

We developed a near infrared (NIR) virtual intraoperative surgical photoacoustic microscopy (NIR-VISPAM) system that combines a conventional surgical microscope and an NIR light photoacoustic microscopy (PAM) system. NIR-VISPAM can simultaneously visualize PA B-scan images at a maximum display rate of 45 Hz and display enlarged microscopic images on a surgeon's view plane through the ocular lenses of the surgical microscope as augmented reality. The use of the invisible NIR light eliminated the disturbance to the surgeon's vision caused by the visible PAM excitation laser in a previous report. Further, the maximum permissible laser pulse energy at this wavelength is approximately 5 times more than that at the visible spectral range. The use of a needle-type ultrasound transducer without any water bath for acoustic coupling can enhance convenience in an intraoperative environment. We successfully guided needle and injected carbon particles in biological tissues ex vivo and in melanoma-bearing mice in vivo.