In vitro maturation and artificial activation of donkey oocytes

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• 作者：Gaoping Zhao^a ; Kaifeng Wu^b ; Liang Cui^b ; Lixia Zhao^b ; Yiyi Liu^b ; Xiuwen Tan^c ; Huanmin Zhou^b ; ^{huanminzhou@263.net"" rel=""nofollow}
• 关键词：Oocyte ; In vitro maturation ; Parthenogenetic activation ; Equus asinus
• 刊名：Theriogenology
• 出版年：2011
• 出版时间：1 September 2011
• 年：2011
• 卷：76
• 期：4
• 页码：700-704
• 全文大小：868 K

文摘

Three media were evaluated for their ability to support in vitro maturation of donkey (Equus asinus) oocytes and their development after parthenogenetic activation. The basal medium for Medium 1 (M1) and Medium 2 (M2) was M199 and DMEM/F12 respectively, whereas, Medium 3 (M3) consisted of equal parts (v/v) of M199 and DMEM/F12. All three media were supplemented with 10 % (v/v) fetal calf serum, 0.01 units/mL porcine FSH, 0.01 units/mL equine LH, 200 ng/mL insulin-like growth factor 1(IGF-I), 10 μl/mL insulin-transferrin-selenium (ITS), 0.1 mg/mL taurine, 0.1 mg/mL L-cysteine, 0.05 mg/mL L-glutamine, 0.11 mg/mL sodium pyruvate, and 25 mg/mL gentamycin. There were no significant differences among the three maturation media for oocyte maturation. Maturation rate of donkey oocytes in M1 was 53 % for compact (Cp) cumulus-oocyte complexes and 75 % for expanded (Ex) cumulus-oocyte complexes; in M2 these were 55 and 77 % , respectively; and in M3, 58 and 75 % . The percentage of cleaved parthenotes and 4- or 8-cell embryos were not significantly different for oocytes matured in the various media (61 and 24 % for M1; 66 and 32 % for M2; and 67 and 33 % for M3). Oocytes matured in M3 tended to yield a higher rate of advanced embryo development (morula) than oocytes matured in M1 (22 vs 9 % ; P = 0.07). In conclusion, donkey oocytes were matured and parthenogenetically activated in vitro, using methods similar to those used in the horse.