A simplified protocol for high-yield expression and purification of bacterial topoisomerase I
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- 作者：Jesse A. Jones ; Emily Price ; Donovan Miller ; Kirk E. Hevener ; khevener@pharmacy.isu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Type IA topoisomerase ; Topoisomerase I ; Streptococcus mutans ; Purification ; Characterization ; Autoinduction
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：124
- 期：Complete
- 页码：32-40
- 全文大小：1051 K
文摘
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Topoisomerase I is a potentially novel and selective antibacterial target.
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A method of purifying Streptococcus mutans topoisomerase I to high yield is presented.
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The use of an auto-induction method for protein expression improved yield.
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>95% purity is achieved in just over 48 h with a simple two-step protocol.