Bivalent peptides as PDZ domain ligands

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• 作者：Edvin Klosi ; Dorina Saro ; Mark R. Spaller
• 关键词：Peptide ; Protein ; ITC ; Calorimetry ; Multivalent ; Bivalent ; PDZ
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2007
• 出版时间：15 November 2007
• 年：2007
• 卷：17
• 期：22
• 页码：6147-6150
• 全文大小：284 K

文摘

A series of multivalent peptides, with the ability to simultaneously bind two separate PDZ domain proteins, has been designed, synthesized, and tested by isothermal titration calorimetry (ITC). The monomer sequences, linked with succinate, varied in length from five to nine residues. The thermodynamic binding parameters, in conjunction with results from mass spectrometry, indicate that a ternary complex is formed in which each peptide arm binds two equivalents of the third PDZ domain (PDZ3) of the neuronal protein PSD-95.