Processing of DNA structures via DNA unwinding and branch migration by the S. cerevisiae Mph1 protein

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• 作者：Xiao-Feng Zheng ; Rohit Prakash¹ ; Dorina Saro ; Simonne Longerich ; Hengyao Niu ; Patrick Sung  ; ;   ; ^{Patrick.Sung@yale.edu}
• 关键词：Mph1 ; Helicase ; Recombination ; D-loop ; Replication fork ; Fork regression ; Branch migration
• 刊名：DNA Repair
• 出版年：2011
• 出版时间：10 October 2011
• 年：2011
• 卷：10
• 期：10
• 页码：1034-1043
• 全文大小：1271 K

文摘

The budding yeast Mph1 protein, the putative ortholog of human FANCM, possesses a 3?to 5?DNA helicase activity and is capable of disrupting the D-loop structure to suppress chromosome arm crossovers in mitotic homologous recombination. Similar to FANCM, genetic studies have implicated Mph1 in DNA replication fork repair. Consistent with this genetic finding, we show here that Mph1 is able to mediate replication fork reversal, and to process the Holliday junction via DNA branch migration. Moreover, Mph1 unwinds 3?and 5?DNA Flap structures that bear key features of the D-loop. These biochemical results not only provide validation for a role of Mph1 in the repair of damaged replication forks, but they also offer mechanistic insights as to its ability to efficiently disrupt the D-loop intermediate.