Simultaneous determination of diphenylamine and nitrosodiphenylamine by photochemically induced fluorescence and synchronous fluorimetry using double scans method

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• 作者：Naader Alizadeh ; ^{alizaden@modares.ac.ir" class="auth_mail} ; Alireza Farokhcheh
• 关键词：Synchronous fluorimetry ; Double scans ; Photochemically induced fluorescence ; Nitrosodiphenylamine ; Diphenylamine
• 刊名：Talanta
• 出版年：April, 2014
• 年：2014
• 卷：121
• 期：Complete
• 页码：239-246
• 全文大小：1492 K

文摘

A new synchronous fluorimetry in the combination with the photochemically induced fluorescence (PIF) method for simultaneous determination of nitrosodiphenylamine (NDPhA) and diphenylamine (DPhA) in aqueous and methanolic solution has been proposed. DPhA has a variety of applications and NDPhA is carcinogenic product of its. The method is based on the use of UV irradiation to produce fluorescent derivatives from NDPhA as a non-fluorescent molecule. The PIF properties of these amines in three media (water, methanol and acetonitrile) are reported. Because of their similar features, the fluorescence emission spectra of NDPhA photoproducts and DPhA were found to severely overlap in the whole wavelength region. Overlapping of conventional fluorescence spectra of these molecules is resolved by synchronous fluorometry using double scans method (SF-DS), thus making the use of separation techniques unnecessary for simultaneous determination of NDPhA and DPhA. The synchronous fluorescence intensity of NDPhA was measured at 螖位 of 127 nm and at 螖位=75 nm for DPhA in solution, which are independent of each other. The best sensitivity can be achieved in water. The linear ranges for determination of NDPhA and DPhA were 1脳10^鈭? to 6脳10^鈭? mol L^鈭? and 4脳10^鈭? to 9脳10^鈭? mol L^鈭?, and the limits of detection (LOD) were 8脳10^鈭? and 1脳10^鈭? mol L^鈭?, respectively. The relative standard deviations (R.S.D.) of method is<3% (n=5). The proposed method was successfully applied for the determination of the two compounds in synthetic solutions, well water samples and also in gunpowder samples. The results obtained were favorably compared to those obtained with HPLC analysis.